Genes, Development And Behavior
Department of Translational Neuroscience
Utrecht, The Netherlands

BCRM iPSC Training Facility

The use and generation of induced pluripotent stem cell (iPS)-generated cell lines has increased exponentially since the first successful reprogramming of human fibroblasts into fully pluripotent iPS cell lines by Yamanaka and colleagues in 2007. Currently, a wide variety of potential applications of iPSC lines is being explored and specific models for disease and developmental processes are being generated. The possibility of generating patient-derived iPSC lines followed by differentiation into desired cell types has the promise to provide new, groundbreaking insights into disease pathology, genetic etiologies and can facilitate the development of therapeutic approaches.

The iPSC Training Facility at the Brain Center Rudolf Magnus provides researchers with the opportunity to develop and execute the core skills and competences required to successfully reprogram patient cells into pluripotent, well-characterized iPSC lines. While conducting the necessary biological techniques themselves, guest researchers will be guided through the process and supplied with the required knowledge and basic materials. In addition to the reprogramming platform, the possibility to differentiate the obtained cell lines into neural lineages is also being offered in the facility and can provide researchers with new, innovative tools to expand their already existing scientific toolbox.

The iPSC Training Facility is located in the Stratenum building of the UMC Utrecht within the Department of Translational Neuroscience. It benefits from the expertise of the Geijsen (Hubrecht) and Pasterkamp (Department of Translational Neuroscience) groups in iPSC technology and neuronal differentiation, respectively. If you are interested in starting an iPSC project in our facility please contact us for more information.

Services

Reprogramming
At the iPSC Training Facility we can assist you in reprogramming patient fibroblasts into iPSC lines  using lentiviral vectors and with expansion of these cell lines on feeder plates. All cell lines will be tested for mycoplasma upon entering our facility and from than on every other week.

Characterization
Following generation of iPSC lines they can be characterized for the expression of pluripotency markers using immunohistochemistry and qPCR.

Cryopreservation
The iPSC Training Facility offers cryopreservation for all iPSC cell lines that are generated in the facility.

Clone maintenance
After reprogramming, the required iPSC clones of the iPSC lines can be maintained in culture.

Neuronal differentiation
At the iPSC Training Facility we can assist in the neuronal differentiation of iPSC lines into specific types of neural cells, such as motor neurons or glia.

If you would like to start an iPSC project at our facility, please fill in the project application form and submit it by email to Inge Dokter.

Contact information:

Inge Dokter
iPSC Facility Manager
E: i.dokter-2@umcutrecht.nl
T: +31 (0) 88 7568429

Postal  address:
BCRM iPSC Training Facility
UMC Utrecht
Str. 4.205
P.O. Box 85060
3508 AB Utrecht
The Netherlands

Visiting address:
BCRM iPSC Training Facility
UMC Utrecht
Room Str. 4.239
Universiteitsweg 100
3584 CG Utrecht
The Netherlands