Prof. dr. Jeroen Pasterkamp

Prof. dr. Jeroen Pasterkamp
PI
I am a molecular neurobiologist trying to dissect the mechanisms that underlie neural circuit development and various neurological and neurodegenerative disorders using a multidisciplinary research approach ranging from in vitro human stem cell modelling and mouse genetics to advanced 3D microscopy.

Marina de Wit

Marina de Wit
Technician
Epilepsy research using human, mouse and rat tissue.
Western blot, in situ hybridization, Qpcr

Youri Adolfs

Youri Adolfs
Technician
I am managing the microscopes of our department as well as the imaging facility of the MIND-Facility. Besides supporting PhD students and Postdocs with 3D immuno-staining procedures and image analysis, I also perform molecular cloning projects and in utero electroporation experiments.
Microscopy (Confocal, Lightsheet), Cloning, In utero electroporation

Daniëlle N. Vonk

Daniëlle N. Vonk
Technician
I am manager of the iPSC/Organoid part of the MIND-Facility.
iPSC culture, Brain organoid culture, differentiations of iPSC into different cell types.

Jasmijn Hundscheid

Jasmijn Hundscheid
Research Technician
I am culturing iPSCs, organoids and motor neurons. The research focuses on modeling cortical thinning in C9ORF72 ALS patients using brain organoids.
PSC culture, brain organoid culture, iPSC derived motor neuron, ALS

Lauri. M. Bloemenkamp

Lauri. M. Bloemenkamp
Technician
Our research focuses on the (pre)clinical development of a novel therapeutic antibody targeting C2.
iPSC culture, iPSC derived motor neurons, complement system

Nicky van Kronenburg

Nicky van Kronenburg
Technician
My aim is to help the researchers with their animal work wherever I can.
mouse breeding, mouse genetics, In utero electroporation

Eljo Y. van Battum

Eljo Y. van Battum
Postdoc
I am a developmental neurobiologist with great interest in axon guidance mechanisms and neuronal network formation.
in vivo electroporation, primary tissue culture, microscopy

Pavol Zelina

Pavol Zelina
Postdoc
My project is aimed to deepen understanding of ALS pathogenesis and to develop a cellular model of the disease.
neurobiology of ALS, iPSC derived motor neurons, axon guidance

Vamshidhar R. Vangoor

Vamshidhar R. Vangoor
Assistant professor
I am a Molecular Neurobiologist interested in understanding the cellular mechanisms involved in neural cell development and in disease processes.
Epilepsy, Animal models of Epilepsy, Non-coding RNAs, Primary cell culture

Domino Schlegel

Domino Schlegel
Postdoc
My aim is to explore the molecular mechanisms causing ALS using iPSC-based disease models, with a particular focus on the role of skeletal muscle
molecular biology, imaging, zebrafish

Suzy Varderidou

Suzy Varderidou
Postdoc
My work focuses on the characterization of disease using iPSC-derived neurons carrying disease associated mutations. I am using mass spectrometry proteomics to identify the proteome alterations of a specific mutation. Furthermore, I study the extracellular vesicles secreated by cells. I am interested to identify whether these vesicles carry disease associated cargo, and whether these vesicles can ‘spread disease’ to unaffected cells.
iPSCs, mass spectrometry proteomics, extracellular vesicles, western blot, microscopy

Astrid T. van der Geest

Astrid T. van der Geest
PhD student
My research focuses on modeling cortical thinning in C9ORF72 ALS patients using brain organoids.
brain organoid culture, ALS, iPSC culture

Marleen H. van den Munkhof

Marleen H. van den Munkhof
PhD student
I focus on exploring the role of Semaphorin-Plexin signalling in neural circuit development.
In utero electroporation, Tissue clearing, Primary cell culture

Rianne de Jongh

Rianne de Jongh
PhD student
My research aims to unravel the role of non-cell autonomous processes in axon degeneration in ALS.
iPSC culture, Microfluidics, ALS

Tijana Ljubikj

Tijana Ljubikj
PhD student
My research focuses on understanding the role of microglia in C9orf72 – ALS using cerebral organoids. I also study the involvment of TDP-43 in ALS using cerebral organoids.
brain organoid culture, microscopy/live cell imaging, iPSC culture and differentiation

Özge Düdükcü

Özge Düdükcü
PhD student
I’m a developmental Neurobiologist and my research focuses on the interaction between GABAergic and Dopaminergic neurons during midbrain development.
Mouse genetics, single cell RNA sequencing, Imaging

Laurens M. Grossouw

Laurens M. Grossouw
PhD student
I am interested in dopaminergic axons in development and disease.
Dopaminergic development, Tissue clearing, Mouse genetics

Oxana Garritsen

Oxana Garritsen
PhD candidate
My research focusses on dissecting the molecular mechanisms involved in the correct positioning of midbrain dopaminergic neurons in health and disease.
Intersectional mouse genetics, tissue clearing, 2D/3D imaging

Marta Cañizares Luna

Marta Cañizares Luna
PhD student
I study the role of Ataxin-2 in ALS using mouse and human models with a particular focus on microglia; I am also interested in developing an organoid system to study microglia in disease, namely ALS and AD.
Brain organoids & iPSC culture, Microglia biology, ALS and AD

Annabel Timmers

Annabel Timmers
PhD candidate
My research aims to study long non-coding RNAs in ALS using iPSC-derived motor neurons.
iPSC culture and differentiation, ALS, non-coding RNA

Channa E. Jakobs

Channa E. Jakobs
PhD student
My work focuses on morphological and synaptic alterations in C9ORF72 ALS patients using brain organoids.
iPSC culture, brain organoids, ALS

Recent Papers

Pasterkamp lab

  1. van de Haar LL, Riga D, Boer JE, Garritsen O, Adolfs Y, Sieburgh TE, van Dijk RE, Watanabe K, van Kronenburg NCH, Broekhoven MH, Posthuma D, Meye FJ, Basak O, Pasterkamp RJ. Molecular signatures and cellular diversity during mouse habenula development. Cell Rep. 2022 Jul 5;40(1):111029. doi: 10.1016/j.celrep.2022.111029. Epub 2016 Dec 8. PMID: 35793630.
  2. Robinson RA, Griffiths SC, van de Haar LL, Malinauskas T, van Battum EY, Zelina P, Schwab RA, Karia D, Malinauskaite L, Brignani S, van den Munkhof MH, Düdükcü Ö, De Ruiter AA, Van den Heuvel DMA, Bishop B, Elegheert J, Aricescu AR, Pasterkamp RJ, Siebold C. Simultaneous binding of Guidance Cues NET1 and RGM blocks extracellular NEO1 signaling. Cell. 2021 Mar 11:S0092-8674(21)00234-8. doi: 10.1016/j.cell.2021.02.045. Epub ahead of print. PMID: 33740419.
  3. Brignani S, Raj DDA, Schmidt ERE, Düdükcü Ö, Adolfs Y, De Ruiter AA, Rybiczka-Tesulov M, Verhagen MG, van der Meer C, Broekhoven MH, Moreno-Bravo JA, Grossouw LM, Dumontier E, Cloutier JF, Chédotal A, Pasterkamp RJ. Remotely Produced and Axon-Derived Netrin-1 Instructs GABAergic Neuron Migration and Dopaminergic Substantia Nigra Development. Neuron. 2020 Aug 19;107(4):684-702.e9. doi: 10.1016/j.neuron.2020.05.037. Epub 2020 Jun 19. PMID: 32562661.
  4. Vangoor VR, Reschke CR, Senthilkumar K, van de Haar LL, de Wit M, Giuliani G, Broekhoven MH, Morris G, Engel T, Brennan GP, Conroy RM, van Rijen PC, Gosselaar PH, Schorge S, Schaapveld RQJ, Henshall DC, De Graan PNE, Pasterkamp RJ. Antagonizing Increased miR-135a Levels at the Chronic Stage of Experimental TLE Reduces Spontaneous Recurrent Seizures. J Neurosci. 2019 Jun 26;39(26):5064-5079. doi: 10.1523/JNEUROSCI.3014-18.2019. Epub 2019 Apr 23. PMID: 31015341; PMCID: PMC6595945.
  5. Ormel PR, Vieira de Sá R, van Bodegraven EJ, Karst H, Harschnitz O, Sneeboer MAM, Johansen LE, van Dijk RE, Scheefhals N, Berdenis van Berlekom A, Ribes Martínez E, Kling S, MacGillavry HD, van den Berg LH, Kahn RS, Hol EM, de Witte LD, Pasterkamp RJ. Microglia innately develop within cerebral organoids. Nat Commun. 2018 Oct 9;9(1):4167. doi: 10.1038/s41467-018-06684-2. PMID: 30301888; PMCID: PMC6177485.
  6. de Jongh R, Spijkers XM, Pasteuning-Vuhman S, Vulto P, Pasterkamp RJ. Neuromuscular junction-on-a-chip: ALS disease modeling and read-out development in microfluidic devices. J Neurochem. 2020 Dec 31. doi: 10.1111/jnc.15289. Epub ahead of print. PMID: 33382092.
WP Twitter Auto Publish Powered By : XYZScripts.com